A Self-Calibrated Single Wavelength Biosensor for Measuring Oxygen Saturation.

Traditional methods for measuring blood oxygen use multiple wavelengths, which produce an intrinsic error due to ratiometric measurements. These methods assume that the absorption changes with the wavelength, but in fact the scattering changes as well and cannot be neglected. We found that if one measures in a specific angle around a cylindrical tissue, called the iso-pathlength (IPL) point, the reemitted light intensity is unaffected by the tissue's scattering. Therefore, the absorption can be isolated from the scattering, which allows the extraction of the subject's oxygen saturation. In this work, we designed an optical biosensor for reading the light intensity reemitted from the tissue, using a single light source and multiple photodetectors (PDs), with one of them in the IPL point's location. Using this bio-device, we developed a methodology to extract the arterial oxygen saturation using a single wavelength light source. We proved this method is not dependent on the light source and is applicable to different measurement locations on the body, with an error of 0.5%. Moreover, we tested thirty-eight males and females with the biosensor under normal conditions. Finally, we show the results of measuring subjects in a hypoxic chamber that simulates extreme conditions with low oxygen.

Optical Method for Detection and Classification of Heavy Metal Contaminants in Water Using Iso-pathlength Point Characterization.

Water pollution caused by hazardous substances, particularly heavy metal (HM) ions, poses a threat to human health and the environment. Traditional methods for measuring HM in water are expensive and time-consuming and require extensive sample preparation. Therefore, developing robust, simple, and sensitive techniques for the detection and classification of HM is needed. We propose an optical approach that exploits the full scattering profile, meaning the angular intensity distribution, and utilizes the iso-pathlength (IPL) point. This point appears where the intensity is constant for different scattering coefficients, while the absorption coefficient is set. The absorption does not affect the IPL point position, it only reduces its intensity. In this paper, we explore the wavelength influence on the IPL point both in Monte Carlo simulations and experimentally. Next, we present the characterization of ferric chloride (FeCl2) by this phenomenon. Eventually, we exhibit the detection of FeCl2 and intralipid mixed in concentrations of 50-100 and 20-30 ppm, respectively. These findings endorse the idea that the IPL point is an intrinsic parameter of a system serving as an absolute calibration point. The method provides an efficient way of differentiating contamination in water. Its characterization technique is easy, precise, and versatile making it preferable for water monitoring.

Detecting Contaminants in Water Based on Full Scattering Profiles within the Single Scattering Regime.

Clean water is essential for maintaining human health. To ensure clean water, it is important to use sensitive detection methods that can identify contaminants in real time. Most techniques do not rely on optical properties and require calibrating the system for each level of contamination. Therefore, we suggest a new technique to measure water contamination using the full scattering profile, which is the angular intensity distribution. From this, we extracted the iso-pathlength (IPL) point which minimizes the effects of scattering. The IPL point is an angle where the intensity values remain constant for different scattering coefficients while the absorption coefficient is set. The absorption coefficient does not affect the IPL point but only attenuates its intensity. In this paper, we show the appearance of the IPL in single scattering regimes for small concentrations of Intralipid. We extracted a unique point for each sample diameter wherein light intensity remained constant. The results describe a linear dependency between the angular position of the IPL point and the sample diameter. In addition, we show that the IPL point separates the absorption from the scattering, which allows the absorption coefficient to be extracted. Eventually, we present how we used the IPL point to detect the contamination levels of Intralipid and India ink in concentrations of 30-46 and 0-4 ppm, respectively. These findings suggest that the IPL point is an intrinsic parameter of a system that can be used as an absolute calibration point. This method provides a new and efficient way of measuring and differentiating between various types of contaminants in water.